Composite

Part:BBa_K4852048:Design

Designed by: Shen, Sean Chuan-En   Group: iGEM23_GEMS-Taiwan   (2023-10-12)


The metJ construct regulate production of biofilm.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2776
    Illegal PstI site found at 2917
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 207
    Illegal NheI site found at 230
    Illegal NheI site found at 2561
    Illegal NheI site found at 2584
    Illegal NheI site found at 3105
    Illegal NheI site found at 3128
    Illegal PstI site found at 2776
    Illegal PstI site found at 2917
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1863
    Illegal XhoI site found at 2418
    Illegal XhoI site found at 3948
    Illegal XhoI site found at 4063
    Illegal XhoI site found at 4618
    Illegal XhoI site found at 4702
    Illegal XhoI site found at 5257
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2776
    Illegal PstI site found at 2917
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2776
    Illegal PstI site found at 2917
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 2848
    Illegal AgeI site found at 1331
    Illegal AgeI site found at 2238
    Illegal AgeI site found at 4438
    Illegal AgeI site found at 5077
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1866
    Illegal BsaI.rc site found at 3951
    Illegal BsaI.rc site found at 4066
    Illegal BsaI.rc site found at 4705


Design Notes

Gibson Assembly


Source

E. coli

References